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產(chǎn)品名稱: 超濾離心管
產(chǎn)品型號(hào): UFC901096
產(chǎn)品展商: 密理博
產(chǎn)品文檔: 無(wú)相關(guān)文檔
簡(jiǎn)單介紹
Millipore超濾管，15ml 10K
超濾離心管
的詳細(xì)介紹
產(chǎn)品名稱 :Millipore超濾管
品牌 :密理博
貨號(hào) :UFC901096
規(guī)格 :15ml 10K
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Amicon Ultra-4* and -15* Centrifugal Filter Units are designed for in vitro diagnostic use and intended for concentrating serum, urine, cerebrospinal fluid and other body fluids prior to analysis.
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The premier devices for concentrating 1 – 4mL or <15 mL
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High recovery Ultracel regenerated cellulose membrane in a range of molecular weight cut-offs
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High retentate recovery of >90%
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Vertical membrane reduces concentration polarization for ultra-fast spin times (as fast as 10–15 minutes)
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Heat-sealed membrane minimizes downstream extractables
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100% integrity tested for reliable performance
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Convenient sample monitoring with translucent housing and volume gradations
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Direct pipettor sample access eliminates processing step to recover concentrate
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High concentration factors of 80–100X
The Amicon Ultra-4 and Amicon Ultra-15 with Ultracel-10 membrane are CE marked.
Applications
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Concentration of biological samples containing antigens, antibodies, enzymes, nucleic acids, or microorganisms
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Purification of macromolecular components found in tissue culture extracts or cell lysates
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Concentration of dilute or pre-purified proteins from column eluents
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Desalting and buffer exchange
Performance data - Amicon Ultra-4 Centrifugal Filter Unit
Typical Spin Times to Filtrate Volume
Spin conditions: 4000 xg swinging bucket rotor at 25 ° C, 4 mL sample.
3K, 10K: Cytochrome c (0.25 mg/mL); 30K, 50K: BSA (1 mg/mL); 100K: IgG (1 mg/mL).
Typical Protein Recovery
Membrane
NMWL, kDa
|
Protein Solute
|
Protein Retentate
Volume, mL
|
Protein Retentate
Recovery, %
|
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10
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Cytochrome c, 12.4 kDa
(0.25 mg/mL)
|
0.20
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95
|
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4 mL starting volume in a fixed angle rotor at 7,500 x g at 25 °C. Spin times: 3K (30 min); 10 and 30K (10 min); 50K (5 min); 100K (15 min).
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Performance data - Amicon Ultra-15 Centrifugal Filter Unit
Typical Spin Times to Filtrate Volume
Spin conditions: 4000 xg swinging bucket rotor at 25° C, 15 mL sample.
3K, 10K: Cytochrome c (0.25 mg/mL); 30K, 50K: BSA (1 mg/mL); 100K: IgG (1 mg/mL).
Typical Protein Recovery
Membrane
NMWL, kDa
|
Protein Solute
|
Protein Retentate
Volume, mL
|
Protein Retentate
Recovery, %
|
|
10
|
Cytochrome c, 12.4 kDa
(0.25 mg/mL)
|
0.24
|
93.03
|
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15 mL starting volume in swinging bucket rotor at 4000 x g at 25 °C. Spin times: 3K (40 min); 10K and 100K (20 min); 30K (10 min); 50K (15 min).
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