膠原蛋白酶來源于溶組織梭菌

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產(chǎn)品名稱: 膠原蛋白酶來源于溶組織梭菌

產(chǎn)品型號: V900892

產(chǎn)品展商: sigma

產(chǎn)品價格: 0.00 元

會員價格: 0.00 元

產(chǎn)品文檔: 無相關(guān)文檔

簡單介紹

產(chǎn)品名稱：膠原蛋白酶來源于溶組織梭菌英文名稱：Collagenase from Clostridium histolyticum Type II, Vetec? reagent grade, powder, ≥125 CDU/mg solid 產(chǎn)品編號 V900892 | CAS號 9001-12-1 | VETEC 規(guī)格：100mg 1g 膠原蛋白酶來源于溶組織梭菌

膠原蛋白酶來源于溶組織梭菌 的詳細(xì)介紹

膠原蛋白酶來源于溶組織梭菌膠原蛋白酶來源于溶組織梭菌膠原蛋白酶來源于溶組織梭菌

Biochem/physiol Actions

Collagenase is activated by four gram atom calcium per mole enzyme. It is inhibited by ethylene glycol-bis(beta-aminoethyl ether) - N, N, N′,N′-tetraacetic acid, beta-mercaptoethanol, glutathione, thioglycolic acid and 8-hydroxyquinoline.

Quality

也具有梭菌蛋白酶、非特異***蛋白酶和胰蛋白酶活性。

Unit Definition

One collagen digestion unit (CDU) liberates peptides from collagen from bovine achilles tendon equivalent in ninhydrin color to 1.0 μmole of leucine in 5 hours at pH 7.4 at 37 °C in the presence of calcium ions. One FALGPA hydrolysis unit hydrolyzes 1.0 μmole of furylacryloyl-Leu-Gly-Pro-Ala per min at 25°C. One Neutral Protease unit hydrolyzes casein to produce color equivalent to 1.0 μmole of tyrosine per 5 hr at pH 7.5 at 37°C. One Clostripain Unit hydrolyzes 1.0 μmole of BAEE per min at pH 7.6 at 25°C in the presence of DTT.

Application

Collagenase from Clostridium histolyticum may be used for the isolation of cells from many types of animal tissue. VETEC Collagenase Type II has higher clostripain activity, and is suggested for bone, heart, liver, thyroid and salivary primary cell isolations.